![]() Our data suggest that cell synchronization activates a significant number of inchoate origins. While only 13.2% and 14.0% of genes within the ENCODE regions are actually transcribed in HeLa and GM06990 cells, 54.5% and 25.6% of zonal origin fragments overlap transcribed genes, most with activating chromatin marks in their promoters. Interestingly, initiation events are distributed about equally between genic and intergenic template sequences. ![]() ![]() Approximately half of the origin fragments cluster into zones, and their signals are generally higher than those of isolated fragments. When hybridized to ENCODE tiling arrays, these libraries illuminated 15.3%, 16.4%, and 21.8% of the genome in the ENCODE regions, respectively. We have used a novel bubble-trapping procedure to construct nearly pure and comprehensive human origin libraries from early S- and log-phase HeLa cells, and from log-phase GM06990, a karyotypically normal lymphoblastoid cell line.
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